Hotspot

ndhC-trnM-CAU

LSC · 54212-55708 · 1497 bp

Back to Spyridium

Pi 0.00697

MarkerSeek score 67.90

Primer available yes

Label ndhC-trnM-CAU

Feature Metadata

label_name: ndhC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1497
variable_sites: 62
pi: 0.00697
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAU_p1	TTCGGGTCCTTTGCTAATCG	CCCGCCGTATGAAAGCAATA	50.0	50.0	58.0	58.4	80-1700 bp	1.0	84.1
ndhC-trnM-CAU_p2	GGGGCTAAAACTCCGGAAAT	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.9	58.4	80-1681 bp	1.0	83.1
ndhC-trnM-CAU_p3	TTCGGGTCCTTTGCTAATCG	CCGCCGTATGAAAGCAATAC	50.0	50.0	58.0	57.3	80-1699 bp	1.0	82.1
ndhC-trnM-CAU_p4	CCTTTGCTAATCGGGGCTAA	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.7	58.4	80-1693 bp	1.0	81.5
ndhC-trnM-CAU_p5	TTCGGGTCCTTTGCTAATCG	TTTGAACCAATGACTCCCGC	50.0	50.0	58.0	58.8	80-1715 bp	1.0	81.3