Hotspot

rps4-trnT-UGU

LSC · 49339-49822 · 484 bp

Back to Spyridium

Pi 0.00649

MarkerSeek score 59.20

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 484
variable_sites: 22
pi: 0.00649
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	80-2665 bp	1.0	85.6
rps4-trnT-UGU_p2	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	80-2667 bp	1.0	85.3
rps4-trnT-UGU_p3	CCCCCAGACGACGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	80-2663 bp	1.0	85.2
rps4-trnT-UGU_p4	TAAGATCGCTTCCGGCTCTA	GGTTCGATTCCGATAGCTGG	50.0	55.0	58.0	58.2	80-2624 bp	1.0	85.2
rps4-trnT-UGU_p5	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	80-2633 bp	1.0	84.9