Hotspot

psaA-ycf3

LSC · 44709-45404 · 696 bp

Back to Spyridium

Pi 0.0056

MarkerSeek score 68.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 25
pi: 0.0056
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	80-888 bp	1.0	85.5
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	80-848 bp	1.0	85.1
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TGCCGAGTATTGGAAACAGG	50.0	50.0	57.9	57.9	80-899 bp	1.0	84.9
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AAGATCACAAGGCGTTTCGA	50.0	45.0	57.9	57.8	80-832 bp	1.0	84.7
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGATCACAAGGCGTTTCGAA	50.0	45.0	57.9	57.8	80-831 bp	1.0	84.7