Hotspot

trnT-GGU-psbD

LSC · 34093-35464 · 1372 bp

Back to Spyridium

Pi 0.00535

MarkerSeek score 60.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1372
variable_sites: 55
pi: 0.00535
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1507-1545 bp	1.0	83.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1510-1548 bp	1.0	82.8
trnT-GGU-psbD_p3	GTAAACGGGCCCCTTTAACT	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.8	58.2	1529-1567 bp	1.0	81.8
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	55.0	50.0	58.1	57.6	1506-1544 bp	1.0	81.8
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.1	57.6	1509-1547 bp	1.0	81.6