Hotspot

trnR-UCU-atpA

LSC · 11068-11513 · 446 bp

Back to Spyridium

Pi 0.00867

MarkerSeek score 58.40

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 22
pi: 0.00867
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	GAGGAAGCAGAAGCCCTTTT	47.6	50.0	57.3	58.1	350-2704 bp	1.0	72.8
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TTTACCGAGGAAGCAGAAGC	47.6	50.0	57.3	57.9	356-2710 bp	1.0	72.7
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AGGAAGCAGAAGCCCTTTTG	47.6	50.0	57.3	58.4	349-2703 bp	1.0	70.5
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	47.6	50.0	57.3	58.4	348-2702 bp	1.0	70.5
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	GAGGAAGCAGAAGCCCTTTT	47.6	50.0	56.9	58.1	385-2704 bp	1.0	69.8