Hotspot

rps16-trnQ-UUG

LSC · 6300-7291 · 992 bp

Back to Spyridium

Pi 0.00713

MarkerSeek score 59.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 992
variable_sites: 53
pi: 0.00713
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GTCGAATAGAAATGGGGCGT	50.0	50.0	57.9	58.1	292-1143 bp	1.0	87.0
rps16-trnQ-UUG_p2	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	293-1083 bp	1.0	87.0
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GTCGAATAGAAATGGGGCGT	45.0	50.0	58.1	58.1	275-1126 bp	1.0	86.6
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	276-1066 bp	1.0	86.6
rps16-trnQ-UUG_p5	CAACGGATCGTGTCCTTCAA	GTCGAATAGAAATGGGGCGT	50.0	50.0	58.2	58.1	293-1144 bp	1.0	86.0