Hotspot

rpl32-trnL-UAG

SSC · 117798-118717 · 920 bp

Back to Spuriopimpinella

Pi 0.095
MarkerSeek score 83.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
920
variable_sites
84
pi
0.095
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTAAGGGATATTGGGCAGC AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 1051-1063 bp 1.0 88.8
rpl32-trnL-UAG_p2 GTAAGGGATATTGGGCAGCC AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 1050-1062 bp 1.0 88.8
rpl32-trnL-UAG_p3 GTAAGGGATATTGGGCAGCC CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 1051-1063 bp 1.0 88.8
rpl32-trnL-UAG_p4 GGTAAGGGATATTGGGCAGC CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 1052-1064 bp 1.0 88.7
rpl32-trnL-UAG_p5 GTAAGGGATATTGGGCAGCC CTCGCACTGCTTCCTAAGAG 55.0 55.0 58.1 58.1 1071-1083 bp 1.0 88.0