Hotspot

trnS-GGA-rps4

LSC · 46255-46566 · 312 bp

Back to Spuriopimpinella

Pi 0.0567

MarkerSeek score 79.80

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 312
variable_sites: 17
pi: 0.0567
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	ATTATTCGCGCCAGACCTAA	50.0	45.0	58.1	57.1	364-372 bp	1.0	78.3
trnS-GGA-rps4_p2	AGGCTTTTGTTTACCGAGGG	ATATTATTCGCGCCAGACCT	50.0	45.0	58.1	56.9	366-374 bp	1.0	76.5
trnS-GGA-rps4_p3	GGCTTTTGTTTACCGAGGGT	ATTATTCGCGCCAGACCTAA	50.0	45.0	58.4	57.1	363-371 bp	1.0	76.0
trnS-GGA-rps4_p4	ATGTAGGAGAGATGGCCGAG	ATTATTCGCGCCAGACCTAA	55.0	45.0	58.4	57.1	416-424 bp	1.0	75.3
trnS-GGA-rps4_p5	TTCACTCGGTTTTTGGGTCA	ATTATTCGCGCCAGACCTAA	45.0	45.0	57.6	57.1	444-452 bp	1.0	74.6