Hotspot

trnT-GGU-psbD

LSC · 32203-33606 · 1404 bp

Back to Spuriopimpinella

Pi 0.0653

MarkerSeek score 83.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1404
variable_sites: 89
pi: 0.0653
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AAGGGCCCTTTTAACTCAGC	AGACCGGACCATCCTACAAA	50.0	50.0	58.1	58.1	1587-1623 bp	1.0	85.5
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	AGACCGGACCATCCTACAAA	50.0	50.0	57.9	58.1	1583-1619 bp	1.0	85.2
trnT-GGU-psbD_p3	AAGGGCCCTTTTAACTCAGC	GAGCAATAGACCGGACCATC	50.0	55.0	58.1	57.8	1594-1630 bp	1.0	84.6
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	GAGCAATAGACCGGACCATC	50.0	55.0	57.9	57.8	1590-1626 bp	1.0	84.4
trnT-GGU-psbD_p5	AAGGGCCCTTTTAACTCAGC	AGAGCAATAGACCGGACCAT	50.0	50.0	58.1	58.2	1595-1631 bp	1.0	84.2