Hotspot

petN-psbM

LSC · 29068-30197 · 1130 bp

Back to Spuriopimpinella

Pi 0.0554

MarkerSeek score 82.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1130
variable_sites: 60
pi: 0.0554
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCTTTTCTGCT	50.0	42.9	58.2	57.2	1272-1283 bp	1.0	71.2
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCTTTTCTGCT	50.0	42.9	57.6	57.2	1211-1222 bp	1.0	69.9
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCTTTTCTGCTT	50.0	40.9	58.2	57.9	1272-1283 bp	1.0	68.7
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCTTTTCTGCTT	50.0	40.9	57.6	57.9	1211-1222 bp	1.0	67.4
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	GTTCCTACTGCTTTTCTGCT	50.0	45.0	58.2	55.7	1271-1282 bp	1.0	66.8