Hotspot

trnH-GUG-psbA

LSC · 79-263 · 185 bp

Back to Spuriopimpinella

Pi 0.138

MarkerSeek score 78.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 25
pi: 0.138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	252-271 bp	1.0	87.3
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	266-285 bp	1.0	86.8
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	265-284 bp	1.0	86.8
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	267-286 bp	1.0	86.8
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.5	57.9	281-300 bp	1.0	83.6