primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	79	263	252-271	1.000000	87.3
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	79	263	266-285	1.000000	86.8
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	79	263	265-284	1.000000	86.8
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	79	263	267-286	1.000000	86.8
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CGCATGGTGGATTCACAATC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.5	57.9	6.4	13.4	0.546122	79	263	281-300	1.000000	83.6
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	TAAAGACGGTTTTCGGTGCT	CTAGTTCCGGGTTCGAATCC	20	20	45.0	55.0	57.8	57.8	0.0	18.0	0.464081	1326	1540	317-332	1.000000	85.7
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	GTAAAGACGGTTTTCGGTGC	CTAGTTCCGGGTTCGAATCC	20	20	50.0	55.0	57.7	57.8	0.0	18.0	0.525731	1326	1540	318-333	1.000000	85.1
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	TACAGAAGCGACCCCATAGG	CTAGTTCCGGGTTCGAATCC	20	20	55.0	55.0	58.6	57.8	0.0	18.0	0.810474	1326	1540	286-301	1.000000	83.8
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	TTTTCGGTGCTGGTTATCCA	CTAGTTCCGGGTTCGAATCC	20	20	45.0	55.0	57.4	57.8	0.0	18.0	0.790894	1326	1540	308-323	1.000000	83.3
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	TAAAGACGGTTTTCGGTGCT	ACTCCATCCGACTAGTTCCG	20	20	45.0	55.0	57.8	58.6	0.0	0.0	0.853037	1326	1540	328-343	1.000000	82.2
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TGCTTTCTACCACAGCGTTT	GGGTTTTGGTCCCGGTATTC	20	20	45.0	55.0	58.0	58.5	0.0	0.0	0.571350	5720	6957	1321-1336	1.000000	82.8
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TTGCTTTCTACCACAGCGTT	GGGTTTTGGTCCCGGTATTC	20	20	45.0	55.0	58.0	58.5	0.0	0.0	0.571350	5720	6957	1322-1337	1.000000	82.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	20	20	45.0	55.0	58.1	58.5	8.1	0.0	0.672468	5720	6957	1332-1347	1.000000	82.0
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TGCTTTCTACCACAGCGTTT	TCGGAGGTTCGAATCCTTTC	20	20	45.0	50.0	58.0	57.3	0.0	16.9	0.705680	5720	6957	1303-1318	1.000000	81.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TTGCTTTCTACCACAGCGTT	TCGGAGGTTCGAATCCTTTC	20	20	45.0	50.0	58.0	57.3	0.0	16.9	0.705680	5720	6957	1304-1319	1.000000	81.8
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.0	57.9	1.4	21.3	0.104185	13463	14638	1199-1238	1.000000	87.0
atpH-atpI_p2	atpH-atpI	2	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	20	20	50.0	55.0	57.9	57.9	0.0	21.3	0.178077	13463	14638	1255-1294	1.000000	86.0
atpH-atpI_p3	atpH-atpI	3	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	57.9	57.9	0.0	21.3	0.214244	13463	14638	1215-1254	1.000000	86.0
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.2	57.9	1.4	21.3	0.249724	13463	14638	1200-1239	1.000000	85.8
atpH-atpI_p5	atpH-atpI	5	GCAGTACCTTGACCAACTCC	ATAGGGGAATCCATGGAGGG	20	20	55.0	55.0	58.2	57.9	0.0	21.3	0.256114	13463	14638	1259-1298	1.000000	85.4
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCTTTTCTGCT	20	21	50.0	42.9	58.2	57.2	0.0	0.0	1.930302	29068	30197	1272-1283	1.000000	71.2
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCTTTTCTGCT	20	21	50.0	42.9	57.6	57.2	0.0	0.0	2.129816	29068	30197	1211-1222	1.000000	69.9
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCTTTTCTGCTT	20	22	50.0	40.9	58.2	57.9	0.0	0.0	2.243464	29068	30197	1272-1283	1.000000	68.7
petN-psbM_p4	petN-psbM	4	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCTTTTCTGCTT	20	22	50.0	40.9	57.6	57.9	0.0	0.0	2.442978	29068	30197	1211-1222	1.000000	67.4
petN-psbM_p5	petN-psbM	5	TAGTAAGTCTCGCTTGGGCT	GTTCCTACTGCTTTTCTGCT	20	20	50.0	45.0	58.2	55.7	0.0	0.0	2.479658	29068	30197	1271-1282	1.000000	66.8
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	31380	32130	855-855	1.000000	89.0
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.273369	31380	32130	820-820	1.000000	88.4
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	31380	32130	852-852	1.000000	87.8
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CGTTGCCTCCTTGAAAGAGA	TTGAACCGATGACTTACGCC	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.452638	31380	32130	857-857	1.000000	86.7
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.4	58.0	0.0	0.0	0.561703	31380	32130	890-890	1.000000	85.6
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	AAGGGCCCTTTTAACTCAGC	AGACCGGACCATCCTACAAA	20	20	50.0	50.0	58.1	58.1	32.5	9.5	0.128284	32203	33606	1587-1623	1.000000	85.5
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GCCCTTTTAACTCAGCGGTA	AGACCGGACCATCCTACAAA	20	20	50.0	50.0	57.9	58.1	0.0	9.5	0.156553	32203	33606	1583-1619	1.000000	85.2
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	AAGGGCCCTTTTAACTCAGC	GAGCAATAGACCGGACCATC	20	20	50.0	55.0	58.1	57.8	32.5	9.5	0.231612	32203	33606	1594-1630	1.000000	84.6
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GCCCTTTTAACTCAGCGGTA	GAGCAATAGACCGGACCATC	20	20	50.0	55.0	57.9	57.8	0.0	9.5	0.259880	32203	33606	1590-1626	1.000000	84.4
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	AAGGGCCCTTTTAACTCAGC	AGAGCAATAGACCGGACCAT	20	20	50.0	50.0	58.1	58.2	32.5	9.5	0.280554	32203	33606	1595-1631	1.000000	84.2
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	45279	46167	906-978	1.000000	78.1
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	45279	46167	927-999	1.000000	77.5
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	21	20	47.6	45.0	58.0	57.8	8.2	0.0	1.233148	45279	46167	877-949	1.000000	77.4
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	21	20	47.6	50.0	58.0	58.3	8.2	0.0	1.301418	45279	46167	879-951	1.000000	76.8
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	21	20	47.6	50.0	58.0	58.4	8.2	0.0	1.427808	45279	46167	907-979	1.000000	75.7
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	AGGCTTTTGTTTACCGAGGG	ATTATTCGCGCCAGACCTAA	20	20	50.0	45.0	58.1	57.1	0.0	0.0	0.997068	46255	46566	364-372	1.000000	78.3
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	AGGCTTTTGTTTACCGAGGG	ATATTATTCGCGCCAGACCT	20	20	50.0	45.0	58.1	56.9	0.0	0.0	1.224897	46255	46566	366-374	1.000000	76.5
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	GGCTTTTGTTTACCGAGGGT	ATTATTCGCGCCAGACCTAA	20	20	50.0	45.0	58.4	57.1	0.0	0.0	1.290028	46255	46566	363-371	1.000000	76.0
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	ATGTAGGAGAGATGGCCGAG	ATTATTCGCGCCAGACCTAA	20	20	55.0	45.0	58.4	57.1	0.0	0.0	1.276019	46255	46566	416-424	1.000000	75.3
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	TTCACTCGGTTTTTGGGTCA	ATTATTCGCGCCAGACCTAA	20	20	45.0	45.0	57.6	57.1	0.0	0.0	1.324456	46255	46566	444-452	1.000000	74.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47605	48373	819-871	1.000000	84.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	47605	48373	851-903	1.000000	83.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ATTAAGTCCGCAGCGTCTAC	20	20	55.0	50.0	57.9	57.8	0.0	15.0	0.338047	47605	48373	837-889	1.000000	83.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ATTAAGTCCGCAGCGTCTAC	20	20	50.0	50.0	57.8	57.8	15.5	15.0	0.445800	47605	48373	869-921	1.000000	82.1
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATGCGATGCTCTAACCTCTG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.527459	47605	48373	821-873	1.000000	81.7
petA-psbJ_p1	petA-psbJ	1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.1	58.1	17.6	0.0	1.201437	63800	64768	1157-1159	1.000000	76.3
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	57.8	58.1	0.0	0.0	1.248500	63800	64768	1144-1146	1.000000	76.0
petA-psbJ_p3	petA-psbJ	3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	58.1	57.8	17.6	0.0	1.293037	63800	64768	1158-1160	1.000000	75.5
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	57.8	57.8	0.0	0.0	1.340100	63800	64768	1145-1147	1.000000	75.2
petA-psbJ_p5	petA-psbJ	5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	20	21	55.0	47.6	57.7	58.1	7.7	0.0	1.404770	63800	64768	1162-1164	1.000000	74.6
psbE-petL_p1	psbE-petL	1	TTACATTGTAGTCGGTGGCG	GGACCAGCCACACAACTAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.025292	68334	69133	1829-1829	0.500000	59.8
psbE-petL_p2	psbE-petL	2	GGAAGGGAAAGTGAGTTGCA	GGACCAGCCACACAACTAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.043084	68334	69133	1702-1702	0.500000	59.7
psbE-petL_p3	psbE-petL	3	TTACATTGTAGTCGGTGGCG	GAATGCGGTCCCTTATTCGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.051486	68334	69133	1593-1593	0.500000	59.6
psbE-petL_p4	psbE-petL	4	TTACATTGTAGTCGGTGGCG	GCGTCTGCTATCATCTCAGG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.064107	68334	69133	1567-1567	0.500000	59.5
psbE-petL_p5	psbE-petL	5	GGAAGGGAAAGTGAGTTGCA	GAATGCGGTCCCTTATTCGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.069278	68334	69133	1466-1466	0.500000	59.4
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GCGTTCTACCGAACTGAACT	CTTGGTAGCGCGTTTGTTTT	20	20	50.0	45.0	57.9	57.9	0.0	8.4	0.175650	70392	70524	220-222	1.000000	83.7
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GCGTTCTACCGAACTGAACT	TTGGTAGCGCGTTTGTTTTG	20	20	50.0	45.0	57.9	58.2	0.0	8.4	0.232908	70392	70524	219-221	1.000000	83.2
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GCGTTCTACCGAACTGAACT	CGCGTTTGTTTTGGGTACAA	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.252065	70392	70524	212-214	1.000000	83.2
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	20	20	50.0	45.0	57.9	57.9	6.2	8.4	0.202860	70392	70524	247-249	1.000000	82.9
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	20	20	50.0	45.0	57.9	58.2	6.2	8.4	0.260118	70392	70524	246-248	1.000000	82.5
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGACGGAAAAAGAAG	ATTCCAGGCTTTTGTCGGTT	20	20	50.0	45.0	58.0	58.0	20.5	0.0	0.037038	116504	117632	1842-1891	1.000000	88.7
ndhF-rpl32_p2	ndhF-rpl32	2	AAGCGGGTCGATCAAGTAAC	AATTCCAGGCTTTTGTCGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.010594	116504	117632	2021-2070	1.000000	88.6
ndhF-rpl32_p3	ndhF-rpl32	3	AAGCGGGTCGATCAAGTAAC	ATTCCAGGCTTTTGTCGGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.010594	116504	117632	2020-2069	1.000000	88.6
ndhF-rpl32_p4	ndhF-rpl32	4	CCGTGTGAACCAAAACCCTA	AATTCCAGGCTTTTGTCGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034532	116504	117632	2249-2298	1.000000	88.1
ndhF-rpl32_p5	ndhF-rpl32	5	CCGTGTGAACCAAAACCCTA	ATTCCAGGCTTTTGTCGGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034532	116504	117632	2248-2297	1.000000	88.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGTAAGGGATATTGGGCAGC	AGCGTGTCTACCGATTTCAC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.096773	117798	118717	1051-1063	1.000000	88.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GTAAGGGATATTGGGCAGCC	AGCGTGTCTACCGATTTCAC	20	20	55.0	50.0	58.1	58.0	1.3	0.0	0.096773	117798	118717	1050-1062	1.000000	88.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GTAAGGGATATTGGGCAGCC	CAGCGTGTCTACCGATTTCA	20	20	55.0	50.0	58.1	58.0	1.3	0.0	0.098143	117798	118717	1051-1063	1.000000	88.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGTAAGGGATATTGGGCAGC	CAGCGTGTCTACCGATTTCA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.098143	117798	118717	1052-1064	1.000000	88.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GTAAGGGATATTGGGCAGCC	CTCGCACTGCTTCCTAAGAG	20	20	55.0	55.0	58.1	58.1	1.3	0.0	0.169024	117798	118717	1071-1083	1.000000	88.0
ycf2_p1	ycf2	1	CAAAGATCTTGAGCGACCGA	TTATCTTTGTTTCCCGGGCC	20	20	50.0	50.0	58.0	58.2	9.2	18.5	0.161657	151230	152229	1840-1840	0.500000	58.7
ycf2_p2	ycf2	2	CAAAGATCTTGAGCGACCGA	CTTACTTCGACAGGGCACAA	20	20	50.0	50.0	58.0	57.8	9.2	0.0	0.167526	151230	152229	2222-2222	0.500000	58.7
ycf2_p3	ycf2	3	CAAAGATCTTGAGCGACCGA	ACGTTGAGAAAGGGCAGATG	20	20	50.0	50.0	58.0	58.2	9.2	0.0	0.190195	151230	152229	2320-2320	0.500000	58.5
ycf2_p4	ycf2	4	CAAAGATCTTGAGCGACCGA	CGTTGAGAAAGGGCAGATGT	20	20	50.0	50.0	58.0	58.2	9.2	0.0	0.190195	151230	152229	2319-2319	0.500000	58.5
ycf2_p5	ycf2	5	ATTTCTTTCATCGGGTCCGG	TTATCTTTGTTTCCCGGGCC	20	20	50.0	50.0	58.0	58.2	9.7	18.5	0.196335	151230	152229	1868-1868	0.500000	58.4