Hotspot

ccsA

SSC · 114569-115555 · 987 bp

Back to Spryginia

Pi 0.00338

MarkerSeek score 33.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 987
variable_sites: 5
pi: 0.00338
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTTTTGAACAAGTTGCCGCT	TTATCGATGGCTTCCCTTGC	45.0	50.0	58.1	58.0	1782 bp	1.0	79.5
ccsA_p2	AAGTTGCCGCTATGGTGAAA	TTATCGATGGCTTCCCTTGC	45.0	50.0	58.1	58.0	1773 bp	1.0	79.4
ccsA_p3	TAGACACGCTGCTCTTAGGA	TTATCGATGGCTTCCCTTGC	50.0	50.0	57.9	58.0	1749 bp	1.0	79.2
ccsA_p4	AGTTGCCGCTATGGTGAAAT	TTATCGATGGCTTCCCTTGC	45.0	50.0	57.9	58.0	1772 bp	1.0	79.1
ccsA_p5	AAATTGGTAGACACGCTGCT	TTATCGATGGCTTCCCTTGC	45.0	50.0	57.8	58.0	1756 bp	1.0	78.7