Hotspot

rpl32-trnL-CUA

SSC · 113571-114372 · 802 bp

Back to Spryginia

Pi 0.0101

MarkerSeek score 58.80

Primer available yes

Label rpl32-trnL-CUA

Feature Metadata

label_name: rpl32-trnL-CUA
feature_type: igs
region: SSC
length_bp: 802
variable_sites: 12
pi: 0.0101
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-CUA_p1	GGAAGGGATATTGGACATCGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.2	57.9	921-936 bp	1.0	67.4
rpl32-trnL-CUA_p2	GGAAGGGATATTGGACATCGT	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.2	57.8	914-929 bp	1.0	66.9
rpl32-trnL-CUA_p3	GGAAGGGATATTGGACATCGT	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.2	57.8	920-935 bp	1.0	66.8
rpl32-trnL-CUA_p4	GGAAGGGATATTGGACATCGT	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.2	57.8	919-934 bp	1.0	66.8
rpl32-trnL-CUA_p5	GGAAGGGATATTGGACATCGT	GAGCAGCGTGTCTACCAATT	47.6	50.0	57.2	58.3	915-930 bp	1.0	66.2