Hotspot

trnP-CCA-psaJ

LSC · 66316-66679 · 364 bp

Back to Spryginia

Pi 0.00366

MarkerSeek score 47.20

Primer available yes

Label trnP-CCA-psaJ

Feature Metadata

label_name: trnP-CCA-psaJ
feature_type: igs
region: LSC
length_bp: 364
variable_sites: 2
pi: 0.00366
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-CCA-psaJ_p1	AAAACAAACGCGCTACCAAG	GGCGCTACGGAAAGATATGT	45.0	50.0	57.9	57.8	427-434 bp	1.0	79.6
trnP-CCA-psaJ_p2	CAAAACAAACGCGCTACCAA	GGCGCTACGGAAAGATATGT	45.0	50.0	58.2	57.8	428-435 bp	1.0	79.1
trnP-CCA-psaJ_p3	TTGTACCCAAAACAAACGCG	GGCGCTACGGAAAGATATGT	45.0	50.0	57.8	57.8	435-442 bp	1.0	78.9
trnP-CCA-psaJ_p4	AAAACAAACGCGCTACCAAG	AGTACTTAGTACCGGCGCTA	45.0	50.0	57.9	57.7	440-447 bp	1.0	78.1
trnP-CCA-psaJ_p5	CAAAACAAACGCGCTACCAA	AGTACTTAGTACCGGCGCTA	45.0	50.0	58.2	57.7	441-448 bp	1.0	77.6