Hotspot

psbJ-psbL

LSC · 63473-63626 · 154 bp

Back to Spryginia

Pi 0.018

MarkerSeek score 64.70

Primer available yes

Label psbJ-psbL

Feature Metadata

label_name: psbJ-psbL
feature_type: igs
region: LSC
length_bp: 154
variable_sites: 4
pi: 0.018
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psbL_p1	ACCGATCACAAGAATACCAGC	ACCAGTCTCTATTGGGGGTT	47.6	50.0	58.1	57.6	286-295 bp	1.0	71.9
psbJ-psbL_p2	CCGATCACAAGAATACCAGCT	ACCAGTCTCTATTGGGGGTT	47.6	50.0	57.8	57.6	285-294 bp	1.0	71.2
psbJ-psbL_p3	TCCTTCCAGTAGTATCAGCCA	ACCAGTCTCTATTGGGGGTT	47.6	50.0	57.6	57.6	239-248 bp	1.0	70.2
psbJ-psbL_p4	CCGATCACAAGAATACCAGC	ACCAGTCTCTATTGGGGGTT	50.0	50.0	56.3	57.6	285-294 bp	1.0	67.2
psbJ-psbL_p5	ACCGATCACAAGAATACCAGC	CGTACCAGTCTCTATTGGGG	47.6	55.0	58.1	56.8	289-298 bp	1.0	65.4