Hotspot

ndhC-trnV-GUA

LSC · 50314-50972 · 659 bp

Back to Spryginia

Pi 0.00202
MarkerSeek score 60.90
Primer available yes
Label ndhC-trnV-GUA

Feature Metadata

label_name
ndhC-trnV-GUA
feature_type
igs
region
LSC
length_bp
659
variable_sites
2
pi
0.00202
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-GUA_p1 TGCTAAAACAGGAATAGCACTTG GTTCGAGTCCGTATAGCCCT 39.1 55.0 57.4 58.7 752-985 bp 1.0 46.2
ndhC-trnV-GUA_p2 ACTCCGGAAATTAGAAATGCT GTTCGAGTCCGTATAGCCCT 38.1 55.0 55.3 58.7 769-1002 bp 1.0 45.4
ndhC-trnV-GUA_p3 TGCTAAAACAGGAATAGCACT GTTCGAGTCCGTATAGCCCT 38.1 55.0 55.2 58.7 752-985 bp 1.0 44.9
ndhC-trnV-GUA_p4 GCTAAAACAGGAATAGCACTTGA GTTCGAGTCCGTATAGCCCT 39.1 55.0 57.1 58.7 751-984 bp 1.0 44.2
ndhC-trnV-GUA_p5 TGCTAAAACAGGAATAGCACTTG TTCGAGTCCGTATAGCCCTA 39.1 50.0 57.4 57.1 751-984 bp 1.0 44.2