Hotspot

ycf3-trnS-UCC

LSC · 43464-44536 · 1073 bp

Back to Spryginia

Pi 0.00186

MarkerSeek score 59.90

Primer available yes

Label ycf3-trnS-UCC

Feature Metadata

label_name: ycf3-trnS-UCC
feature_type: igs
region: LSC
length_bp: 1073
variable_sites: 3
pi: 0.00186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-UCC_p1	GGGTCAATTTCTAGGCGCAT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	58.3	58.1	1217-1221 bp	1.0	76.9
ycf3-trnS-UCC_p2	CCTTCAGATTGAGCCGACAT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.7	58.1	1160-1164 bp	1.0	76.9
ycf3-trnS-UCC_p3	GGGTCAATTTCTAGGCGCAT	TGTTACGCCTTCAACCACTC	50.0	50.0	58.3	58.1	1182-1186 bp	1.0	76.6
ycf3-trnS-UCC_p4	CCTTCAGATTGAGCCGACAT	TGTTACGCCTTCAACCACTC	50.0	50.0	57.7	58.1	1125-1129 bp	1.0	76.6
ycf3-trnS-UCC_p5	GGGTCAATTTCTAGGCGCAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	1238-1242 bp	1.0	76.4