Hotspot

trnC-UGC-petN

LSC · 27218-27887 · 670 bp

Back to Spryginia

Pi 0.000997

MarkerSeek score 59.00

Primer available yes

Label trnC-UGC-petN

Feature Metadata

label_name: trnC-UGC-petN
feature_type: igs
region: LSC
length_bp: 670
variable_sites: 1
pi: 0.000997
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-UGC-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	777-780 bp	1.0	79.7
trnC-UGC-petN_p2	GTTTCTATCGTTTTGGCGGC	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	833-836 bp	1.0	79.5
trnC-UGC-petN_p3	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	778-781 bp	1.0	79.1
trnC-UGC-petN_p4	GTTTCTATCGTTTTGGCGGC	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	834-837 bp	1.0	78.9
trnC-UGC-petN_p5	TTTCCCCAGTTCAAATCCGG	TTCCCCACACTACGAGAGAG	50.0	55.0	58.1	58.2	772-775 bp	1.0	78.4