Hotspot

psbA

LSC · 307-1260 · 954 bp

Back to Sporoxeia

Pi 0.0115

MarkerSeek score 52.70

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 954
variable_sites: 16
pi: 0.0115
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCAAAAACTCGTCTAAATTCAGACT	CATTGGATGGTTTGGTGTTTTG	32.0	40.9	57.0	56.9	1013 bp	1.0	41.3
psbA_p2	TCAAAAACTCGTCTAAATTCAGACT	TTGGATGGTTTGGTGTTTTG	32.0	40.0	57.0	54.9	1011 bp	1.0	41.3
psbA_p3	TCAAAAACTCGTCTAAATTCAGAC	CATTGGATGGTTTGGTGTTTTG	33.3	40.9	55.7	56.9	1013 bp	1.0	41.3
psbA_p4	TCAAAAACTCGTCTAAATTCAGACT	CATTGGATGGTTTGGTGTTT	32.0	40.0	57.0	54.6	1013 bp	1.0	41.3
psbA_p5	CAAAAACTCGTCTAAATTCAGACT	CATTGGATGGTTTGGTGTTTTG	33.3	40.9	55.7	56.9	1012 bp	1.0	41.3