Hotspot

clpP

LSC · 71148-73300 · 2153 bp

Back to Sporoxeia

Pi 0.00852

MarkerSeek score 48.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2153
variable_sites: 27
pi: 0.00852
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AGTTGGCATGTTGAATCGGA	CTGTAATACTCCAACCGCCC	45.0	55.0	57.8	58.1	3000 bp	0.333333	51.3
clpP_p2	GGCATGTTGAATCGGATCCA	CCTGTAATACTCCAACCGCC	50.0	55.0	58.3	58.1	2997 bp	0.333333	50.4
clpP_p3	GGCATGTTGAATCGGATCCA	CTGTAATACTCCAACCGCCC	50.0	55.0	58.3	58.1	2996 bp	0.333333	50.4
clpP_p4	TGGCATGTTGAATCGGATCC	CCTGTAATACTCCAACCGCC	50.0	55.0	58.3	58.1	2998 bp	0.333333	50.4
clpP_p5	TGGCATGTTGAATCGGATCC	CTGTAATACTCCAACCGCCC	50.0	55.0	58.3	58.1	2997 bp	0.333333	50.4