Hotspot

ndhF-rpl32

SSC · 114488-115391 · 904 bp

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Pi 0.0186
MarkerSeek score 61.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
904
variable_sites
24
pi
0.0186
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGGGAAGTGGAATGAGAGGT TCCAAATATTCTTACGAATATGTT 50.0 25.0 58.0 52.3 1008-1052 bp 1.0 42.7
ndhF-rpl32_p2 TGGGAAGTGGAATGAGAGGT TTCCAAATATTCTTACGAATATGT 50.0 25.0 58.0 52.3 1009-1053 bp 1.0 42.7
ndhF-rpl32_p3 TGGGAAGTGGAATGAGAGGTA TCCAAATATTCTTACGAATATGTT 47.6 25.0 57.8 52.3 1008-1052 bp 1.0 42.7
ndhF-rpl32_p4 TGGGAAGTGGAATGAGAGGTA TTCCAAATATTCTTACGAATATGT 47.6 25.0 57.8 52.3 1009-1053 bp 1.0 42.7
ndhF-rpl32_p5 TGTTGCCATTGGAAAAACGA TCCAAATATTCTTACGAATATGTT 40.0 25.0 56.7 52.3 1055-1099 bp 1.0 42.6