Hotspot

petL-petG

LSC · 66712-66892 · 181 bp

Back to Sporoxeia

Pi 0.0516

MarkerSeek score 70.20

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 14
pi: 0.0516
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TCATTTAGCCCCTTCATGCT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	56.9	58.0	623 bp	1.0	73.4
petL-petG_p2	TCATTTAGCCCCTTCATGCT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	56.9	58.0	601 bp	1.0	73.2
petL-petG_p3	TCATTTAGCCCCTTCATGCT	CAAAACCCGATGTCGTAGGT	45.0	50.0	56.9	57.9	587 bp	1.0	72.8
petL-petG_p4	TCATTTAGCCCCTTCATGCT	AAGAAAGGGATGTAGCGCAG	45.0	50.0	56.9	58.0	892 bp	1.0	72.7
petL-petG_p5	TCATTTAGCCCCTTCATGCT	GGGTACAAAATGTCACGGGT	45.0	50.0	56.9	58.1	852 bp	1.0	72.2