Hotspot

trnS-UGA-psbZ

LSC · 36363-36739 · 377 bp

Back to Sporoxeia

Pi 0.0159

MarkerSeek score 53.90

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 377
variable_sites: 9
pi: 0.0159
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	TTTTCAAGACCGGAGCCATC	TGCAAAAACAGCCAATTGGA	50.0	40.0	58.2	57.0	450 bp	1.0	72.2
trnS-UGA-psbZ_p2	AGCAAAAGGAGAGAGAGGGA	TGCAAAAACAGCCAATTGGA	50.0	40.0	57.7	57.0	509 bp	1.0	70.8
trnS-UGA-psbZ_p3	TTTTCAAGACCGGAGCCATC	TGCAAAAACAGCCAATTGGAA	50.0	38.1	58.2	57.7	450 bp	1.0	70.0
trnS-UGA-psbZ_p4	GTTTTCAAGACCGGAGCCAT	TGCAAAAACAGCCAATTGGA	50.0	40.0	58.5	57.0	451 bp	1.0	69.9
trnS-UGA-psbZ_p5	TTTTCAAGACCGGAGCCATC	AACAGCCAATTGGAAAGCAA	50.0	40.0	58.2	56.7	444 bp	1.0	69.8