Hotspot

psbC-trnS-UGA

LSC · 35992-36269 · 278 bp

Back to Sporoxeia

Pi 0.0228
MarkerSeek score 65.70
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
278
variable_sites
9
pi
0.0228
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 AACCGGTTCTTTCCATGACC GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.1 58.2 327-333 bp 1.0 81.1
psbC-trnS-UGA_p2 AACCGGTTCTTTCCATGACC GATGGCTCCGGTCTTGAAAA 50.0 50.0 58.1 58.2 377-383 bp 1.0 81.0
psbC-trnS-UGA_p3 AACCGGTTCTTTCCATGACC ATTCGATTGCGGAGAGATGG 50.0 50.0 58.1 57.8 407-413 bp 1.0 80.9
psbC-trnS-UGA_p4 GGTTCTTTCCATGACCCCTC GATGGCTCCGGTCTTGAAAA 55.0 50.0 57.9 58.2 373-379 bp 1.0 80.7
psbC-trnS-UGA_p5 GGTTCTTTCCATGACCCCTC ATTCGATTGCGGAGAGATGG 55.0 50.0 57.9 57.8 403-409 bp 1.0 80.6