Hotspot

trnY-GUA-trnE-UUC

LSC · 31160-31218 · 59 bp

Back to Sporoxeia

Pi 0.0452

MarkerSeek score 62.40

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 4
pi: 0.0452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	CGGATCAGGGAATTCTTGGG	AAGTATGCCCCCATCGTCTA	55.0	50.0	58.0	57.9	238-265 bp	1.0	80.3
trnY-GUA-trnE-UUC_p2	CGGATCAGGGAATTCTTGGG	TAATATGATGGCGGTTGGGC	55.0	50.0	58.0	58.1	258-285 bp	1.0	80.2
trnY-GUA-trnE-UUC_p3	TCATTTCATGGCGGATCAGG	TAATATGATGGCGGTTGGGC	50.0	50.0	58.0	58.1	269-296 bp	1.0	80.2
trnY-GUA-trnE-UUC_p4	TCATTTCATGGCGGATCAGG	AAGTATGCCCCCATCGTCTA	50.0	50.0	58.0	57.9	249-276 bp	1.0	80.2
trnY-GUA-trnE-UUC_p5	CGGATCAGGGAATTCTTGGG	TCGTCTAGTGGTTCAGGACA	55.0	50.0	58.0	57.7	225-252 bp	1.0	78.9