Hotspot

ccsA-ndhD

SSC · 106045-106236 · 192 bp

Back to Sporobolus

Pi 0.0657

MarkerSeek score 73.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 192
variable_sites: 30
pi: 0.0657
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCAACTCTTTTGTGCGGATC	CTCTCGCTATCCGTTGACAG	50.0	55.0	58.0	57.9	772-787 bp	1.0	85.4
ccsA-ndhD_p2	GCAACTCTTTTGTGCGGATC	TTGCCTTACCAGGAATGAGC	50.0	50.0	58.0	57.9	1062-1077 bp	0.888889	80.0
ccsA-ndhD_p3	GCAACTCTTTTGTGCGGATC	GAATGAGCGGTTTTGTTGCA	50.0	45.0	58.0	57.9	1050-1065 bp	0.888889	79.9
ccsA-ndhD_p4	TTCCGCATTTGTAGCTTCGA	ACCACGCGAACTCTTTCTTT	45.0	45.0	57.9	58.1	437-443 bp	0.666667	71.9
ccsA-ndhD_p5	GCAACTCTTTTGTGCGGATC	ACCACGCGAACTCTTTCTTT	50.0	45.0	58.0	58.1	845-856 bp	0.666667	71.5