Hotspot

ndhF-rpl32

SSC · 103288-104220 · 933 bp

Back to Sporobolus

Pi 0.0417

MarkerSeek score 82.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 933
variable_sites: 103
pi: 0.0417
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGATTACCCAGGCATATTGA	TGACATCGAAGTACGTTTTT	45.0	35.0	54.5	53.2	931-1004 bp	1.0	52.0
ndhF-rpl32_p2	GGATTACCCAGGCATATTGA	TTGACATCGAAGTACGTTTT	45.0	35.0	54.5	53.2	932-1005 bp	1.0	52.0
ndhF-rpl32_p3	GGATTACCCAGGCATATTGA	TTTGACATCGAAGTACGTTT	45.0	35.0	54.5	53.2	933-1006 bp	1.0	52.0
ndhF-rpl32_p4	GGATTACCCAGGCATATTGA	TTTTGACATCGAAGTACGTT	45.0	35.0	54.5	53.2	934-1007 bp	1.0	52.0
ndhF-rpl32_p5	GGATTACCCAGGCATATTGA	TTTTTGACATCGAAGTACGT	45.0	35.0	54.5	53.2	935-1008 bp	1.0	52.0