Hotspot

rpl14-rpl16

LSC · 77131-77235 · 105 bp

Back to Sporobolus

Pi 0.0495

MarkerSeek score 70.30

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 105
variable_sites: 16
pi: 0.0495
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TCAGCAATAGTGTCCTTGCC	ACCGGTTACACTAAGACCCA	50.0	50.0	57.9	58.0	829-847 bp	1.0	84.3
rpl14-rpl16_p2	TCAGCAATAGTGTCCTTGCC	GCAATGACACGATATGCACG	50.0	50.0	57.9	58.0	888-906 bp	1.0	84.1
rpl14-rpl16_p3	TCAGCAATAGTGTCCTTGCC	GCTTGGATTACAGCGAGACA	50.0	50.0	57.9	58.0	927-945 bp	1.0	83.9
rpl14-rpl16_p4	TGATGCCGCAAAGTTGAAAC	GCAATGACACGATATGCACG	45.0	50.0	57.9	58.0	1062-1080 bp	0.777778	74.5
rpl14-rpl16_p5	TGATGCCGCAAAGTTGAAAC	GCTTGGATTACAGCGAGACA	45.0	50.0	57.9	58.0	1101-1119 bp	0.777778	74.4