Hotspot

trnL-UAA-trnF-GAA

LSC · 47132-47483 · 352 bp

Back to Sporobolus

Pi 0.0351

MarkerSeek score 73.70

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 352
variable_sites: 33
pi: 0.0351
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AAGTCGTGAGGGTTCAAGTC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	426-462 bp	1.0	84.7
trnL-UAA-trnF-GAA_p2	AAGTCGTGAGGGTTCAAGTC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	441-477 bp	1.0	84.1
trnL-UAA-trnF-GAA_p3	AAGTCGTGAGGGTTCAAGTC	TGTGCCAGGAACCAGATTTG	50.0	50.0	57.5	58.4	442-478 bp	1.0	81.8
trnL-UAA-trnF-GAA_p4	AAGTCGTGAGGGTTCAAGTC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	423-459 bp	1.0	81.6
trnL-UAA-trnF-GAA_p5	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	414-450 bp	1.0	81.5