Hotspot

ycf3-trnS-GGA

LSC · 43778-44380 · 603 bp

Back to Sporobolus

Pi 0.0326

MarkerSeek score 70.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 603
variable_sites: 51
pi: 0.0326
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTTTTCCCCTGAGGTTGTCG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.0	58.2	747-790 bp	1.0	88.1
ycf3-trnS-GGA_p2	TTTTTCCCCTGAGGTTGTCG	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.0	57.8	697-740 bp	1.0	88.1
ycf3-trnS-GGA_p3	TGAAGCATCAAAGGCCACAT	ACGGAAAGAGAGGGATTCGA	45.0	50.0	58.1	58.2	947-997 bp	1.0	87.3
ycf3-trnS-GGA_p4	AAGCATCAAAGGCCACATCA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	58.1	58.2	945-995 bp	0.777778	77.6
ycf3-trnS-GGA_p5	AGCATCAAAGGCCACATCAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	58.1	58.2	944-994 bp	0.777778	77.6