Hotspot

trnR-UCU-rps14

LSC · 35880-36228 · 349 bp

Back to Sporobolus

Pi 0.0507
MarkerSeek score 76.60
Primer available yes
Label trnR-UCU-rps14

Feature Metadata

label_name
trnR-UCU-rps14
feature_type
igs
region
LSC
length_bp
349
variable_sites
46
pi
0.0507
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-rps14_p1 AGAAGACCTCTGTCCTATCCA GGTGCAACAAGATCTAGTTGG 47.6 47.6 56.9 56.9 397-411 bp 1.0 58.7
trnR-UCU-rps14_p2 AGGTTTAGAAGACCTCTGTCCT GGTGCAACAAGATCTAGTTGG 45.5 47.6 57.9 56.9 403-417 bp 1.0 58.0
trnR-UCU-rps14_p3 AGAAGACCTCTGTCCTATCCA GGTGCAACAAGATCTAGTTGGT 47.6 45.5 56.9 58.6 397-411 bp 1.0 54.9
trnR-UCU-rps14_p4 AGAAGACCTCTGTCCTATCCAT GGTGCAACAAGATCTAGTTGG 45.5 47.6 57.4 56.9 397-411 bp 1.0 54.7
trnR-UCU-rps14_p5 AGGTTTAGAAGACCTCTGTCCT GGTGCAACAAGATCTAGTTGGT 45.5 45.5 57.9 58.6 403-417 bp 1.0 54.2