Hotspot

atpH-atpF

LSC · 32203-32661 · 459 bp

Back to Sporobolus

Pi 0.0379

MarkerSeek score 74.70

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 459
variable_sites: 50
pi: 0.0379
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	TGTGGCACTAGCGCTTTTAT	AAAAACAAAAGAATGGGTTACA	45.0	27.3	57.9	52.9	513-535 bp	1.0	51.0
atpH-atpF_p2	TGTGGCACTAGCGCTTTTAT	AAAACAAAAGAATGGGTTACAT	45.0	27.3	57.9	52.7	512-534 bp	1.0	51.0
atpH-atpF_p3	TTGTGGCACTAGCGCTTTTA	AAAACAAAAGAATGGGTTACAT	45.0	27.3	58.1	52.7	513-535 bp	1.0	51.0
atpH-atpF_p4	TGTGGCACTAGCGCTTTTAT	AAAAACAAAAGAATGGGTTACAT	45.0	26.1	57.9	53.6	513-535 bp	1.0	51.0
atpH-atpF_p5	TTGTGGCACTAGCGCTTTTA	AAAAACAAAAGAATGGGTTACA	45.0	27.3	58.1	52.9	514-536 bp	1.0	50.9