Hotspot

trnD-GUC-psbM

LSC · 15200-16188 · 989 bp

Back to Sporobolus

Pi 0.03
MarkerSeek score 72.30
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
989
variable_sites
83
pi
0.03
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 AGCTCGGCCCAAAAATCTAG GGGAGAAGTGGACTCTAGGG 50.0 60.0 58.0 58.2 1927-2349 bp 1.0 87.6
trnD-GUC-psbM_p2 AGCTCGGCCCAAAAATCTAG GGGGAGAAGTGGACTCTAGG 50.0 60.0 58.0 58.2 1928-2350 bp 1.0 87.6
trnD-GUC-psbM_p3 AGCTCGGCCCAAAAATCTAG GGGGGAGAAGTGGACTCTAG 50.0 60.0 58.0 58.2 1929-2351 bp 1.0 87.6
trnD-GUC-psbM_p4 AATTCTTCCTGGGTCGATGC GGGAGAAGTGGACTCTAGGG 50.0 60.0 58.0 58.2 2010-2432 bp 1.0 87.4
trnD-GUC-psbM_p5 AATTCTTCCTGGGTCGATGC GGGGAGAAGTGGACTCTAGG 50.0 60.0 58.0 58.2 2011-2433 bp 1.0 87.4