Hotspot

trnT-GGU-trnE-UUC

LSC · 14004-14559 · 556 bp

Back to Sporobolus

Pi 0.0307

MarkerSeek score 73.20

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 556
variable_sites: 46
pi: 0.0307
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GCCACTAGCGTATGAACCAA	GCATCGACCCAGGAAGAATT	50.0	50.0	58.0	58.0	1191-1611 bp	1.0	86.8
trnT-GGU-trnE-UUC_p2	GCCACTAGCGTATGAACCAA	CTAGATTTTTGGGCCGAGCT	50.0	50.0	58.0	58.0	1274-1694 bp	1.0	86.5
trnT-GGU-trnE-UUC_p3	GTCAAGGGTAGCCATTCGAG	GCATCGACCCAGGAAGAATT	55.0	50.0	58.1	58.0	1328-1748 bp	1.0	86.4
trnT-GGU-trnE-UUC_p4	GCCACTAGCGTATGAACCAA	TTGAACTACAATCCCTGCGG	50.0	50.0	58.0	57.9	1628-2051 bp	1.0	86.3
trnT-GGU-trnE-UUC_p5	GTCAAGGGTAGCCATTCGAG	CTAGATTTTTGGGCCGAGCT	55.0	50.0	58.1	58.0	1411-1831 bp	1.0	86.1