Hotspot

psbC-trnS-UGA

LSC · 10928-11081 · 154 bp

Back to Sporobolus

Pi 0.0382

MarkerSeek score 71.60

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 154
variable_sites: 14
pi: 0.0382
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	AGCAGGCTTTGAAAAGGGAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	57.9	58.0	301-311 bp	1.0	86.5
psbC-trnS-UGA_p2	CATTTGTGGCATGCAGGAAG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	336-346 bp	1.0	86.4
psbC-trnS-UGA_p3	ATTTGTGGCATGCAGGAAGA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.1	58.0	335-345 bp	1.0	85.9
psbC-trnS-UGA_p4	TTCAACCTTGGCAAGAACGA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.0	58.0	506-516 bp	1.0	84.6
psbC-trnS-UGA_p5	CAGCGTCTTGGAGCTAATGT	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	693-703 bp	1.0	84.2