Hotspot

rbcL

LSC · 60110-61537 · 1428 bp

Back to Spondias

Pi 0.00665

MarkerSeek score 60.70

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 18
pi: 0.00665
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGAGTTGTAGGGAGGGACTT	ATAGAATCGTTGGGTTCGGC	50.0	50.0	57.3	58.1	1541 bp	1.0	74.8
rbcL_p2	TCATGAGTTGTAGGGAGGGA	ATAGAATCGTTGGGTTCGGC	50.0	50.0	57.1	58.1	1544 bp	1.0	73.0
rbcL_p3	CATGAGTTGTAGGGAGGGAC	ATAGAATCGTTGGGTTCGGC	55.0	50.0	57.0	58.1	1543 bp	1.0	72.6
rbcL_p4	TGAGTTGTAGGGAGGGACTT	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.3	58.4	1500 bp	1.0	72.2
rbcL_p5	TGAGTTGTAGGGAGGGACTT	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.3	58.4	1501 bp	1.0	72.2