Hotspot

trnN-GUU-trnR-ACG

SSC · 136971-137574 · 604 bp

Back to Spondias

Pi 0.00442

MarkerSeek score 61.80

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: SSC
length_bp: 604
variable_sites: 5
pi: 0.00442
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGGTTGAGAACGGGAATTG	TAGGGTACTTCCGGGAACAG	50.0	55.0	58.1	58.1	837-843 bp	1.0	78.6
trnN-GUU-trnR-ACG_p2	GCTGTTAACTGACTGGTCGT	TAGGGTACTTCCGGGAACAG	50.0	55.0	57.8	58.1	760-766 bp	1.0	78.2
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	TAGGGTACTTCCGGGAACAG	50.0	55.0	58.2	58.1	747-753 bp	1.0	78.1
trnN-GUU-trnR-ACG_p4	TAGAATCTCCCGCTGTTCCT	TAGGGTACTTCCGGGAACAG	50.0	55.0	57.8	58.1	805-811 bp	1.0	78.1
trnN-GUU-trnR-ACG_p5	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	786-792 bp	1.0	78.0