Hotspot

psbE-petL

LSC · 70041-71441 · 1401 bp

Back to Spondias

Pi 0.00976

MarkerSeek score 60.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1401
variable_sites: 26
pi: 0.00976
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	CGAAGTTATCGTTAAAGCCGC	50.0	47.6	57.9	58.0	1537-1562 bp	1.0	74.2
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	CGAAGTTATCGTTAAAGCCGC	50.0	47.6	57.8	58.0	1460-1485 bp	1.0	73.6
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCA	50.0	45.0	57.9	56.9	1523-1548 bp	1.0	73.5
psbE-petL_p4	CAACCCGCAATGAATAGGGA	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.9	56.9	1522-1547 bp	1.0	73.5
psbE-petL_p5	ACGAATAACCAACCCGCAAT	CGAAGTTATCGTTAAAGCCGC	45.0	47.6	58.2	58.0	1546-1571 bp	1.0	73.3