Hotspot

ycf4-cemA

LSC · 65372-66297 · 926 bp

Back to Spondias

Pi 0.015

MarkerSeek score 55.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 926
variable_sites: 26
pi: 0.015
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGACTCCGCGAGAAATTGAG	TTGTGGAGCCCTATACGACA	50.0	50.0	58.0	58.1	1276-1296 bp	1.0	80.8
ycf4-cemA_p2	TGAGAATTTGACTCCGCGAG	TTGTGGAGCCCTATACGACA	50.0	50.0	58.0	58.1	1284-1304 bp	1.0	80.8
ycf4-cemA_p3	TGACTCCGCGAGAAATTGAG	TGGAGCCCTATACGACACTT	50.0	50.0	58.0	57.8	1273-1293 bp	1.0	80.7
ycf4-cemA_p4	TGAGAATTTGACTCCGCGAG	TGGAGCCCTATACGACACTT	50.0	50.0	58.0	57.8	1281-1301 bp	1.0	80.7
ycf4-cemA_p5	AGGAGGGGGCTGTTCTTATT	TTGTGGAGCCCTATACGACA	50.0	50.0	58.0	58.1	1931-1951 bp	1.0	80.6