Hotspot

atpI-rps2

LSC · 17626-17845 · 220 bp

Back to Spondias

Pi 0.0174

MarkerSeek score 64.30

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 220
variable_sites: 7
pi: 0.0174
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	ATGTTGGCCTACTTCCACAC	GCAATTTGTGAGGGTCGTTC	50.0	50.0	57.8	57.7	337 bp	1.0	77.4
atpI-rps2_p2	TTCCACACCGGATATCTCGT	GCAATTTGTGAGGGTCGTTC	50.0	50.0	58.2	57.7	325 bp	1.0	77.2
atpI-rps2_p3	ATGTTGGCCTACTTCCACAC	TTGTGAGGGTCGTTCTAGCT	50.0	50.0	57.8	58.4	332 bp	1.0	77.1
atpI-rps2_p4	TTCCACACCGGATATCTCGT	TTGTGAGGGTCGTTCTAGCT	50.0	50.0	58.2	58.4	320 bp	1.0	77.0
atpI-rps2_p5	ATGTTGGCCTACTTCCACAC	GATTCCAGCAAACGACGATG	50.0	50.0	57.8	57.6	398 bp	1.0	76.3