Hotspot

matK-trnK-UUU

LSC · 3887-4636 · 750 bp

Back to Spondias

Pi 0.0113

MarkerSeek score 61.60

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 15
pi: 0.0113
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GTCGTGTTGCTGAGATCCAT	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.0	58.2	805-825 bp	1.0	82.0
matK-trnK-UUU_p2	GTCGTGTTGCTGAGATCCAT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.0	58.2	804-824 bp	1.0	82.0
matK-trnK-UUU_p3	AAGTCGTGTTGCTGAGATCC	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.9	58.2	807-827 bp	1.0	81.4
matK-trnK-UUU_p4	AAGTCGTGTTGCTGAGATCC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.9	58.2	806-826 bp	1.0	81.4
matK-trnK-UUU_p5	GGAAGTCGTGTTGCTGAGAT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.9	58.2	808-828 bp	1.0	81.4