Hotspot

trnH-GUG-psbA

LSC · 209-745 · 537 bp

Back to Spondias

Pi 0.0332

MarkerSeek score 80.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 537
variable_sites: 33
pi: 0.0332
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	647-715 bp	1.0	83.6
trnH-GUG-psbA_p2	TTAGTTATGGGCGAACGACG	GCGCTAACCTTGGTATGGAA	50.0	50.0	58.1	58.0	709-777 bp	1.0	83.5
trnH-GUG-psbA_p3	TTAGTTATGGGCGAACGACG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.1	57.9	662-730 bp	1.0	83.5
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	661-729 bp	1.0	83.4
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	660-728 bp	1.0	83.4