Hotspot

rps8

LSC · 3451-3861 · 411 bp

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Pi 0

MarkerSeek score 21.40

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 411
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 21.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	CAAAGGCGACGACGGTATAA	TTGACCCTATCCCCCATCAG	50.0	55.0	58.1	58.2	968-986 bp	1.0	80.9
rps8_p2	CATAGGAGCCGCTGGTAATC	TTGACCCTATCCCCCATCAG	55.0	55.0	57.9	58.2	1106-1124 bp	1.0	80.7
rps8_p3	CATAGGAGCCGCTGGTAATC	GGGTAGGGCTTCCGTAACTA	55.0	55.0	57.9	58.2	999-1017 bp	1.0	80.6
rps8_p4	CAAAGGCGACGACGGTATAA	TTGGACATTTCCGTGGGTAC	50.0	50.0	58.1	57.8	1387-1400 bp	1.0	79.9
rps8_p5	CATAGGAGCCGCTGGTAATC	TTGGACATTTCCGTGGGTAC	55.0	50.0	57.9	57.8	1525-1538 bp	1.0	79.8