Hotspot

rpl32-ndhF

SSC · 113504-114343 · 840 bp

Back to Spodiopogon

Pi 0.00905

MarkerSeek score 44.20

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 840
variable_sites: 15
pi: 0.00905
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ATTTCACCATGGCGGCTAAT	CTGGAAGTGGGAGAAGAGGA	45.0	55.0	57.9	58.1	1619-1645 bp	1.0	80.6
rpl32-ndhF_p2	TCGTTGCCGCTAGAAAATGA	CTGGAAGTGGGAGAAGAGGA	45.0	55.0	57.9	58.1	1026-1046 bp	1.0	80.3
rpl32-ndhF_p3	TCCTAAGAGCAGCGTGTCTA	CTGGAAGTGGGAGAAGAGGA	50.0	55.0	57.9	58.1	1642-1668 bp	1.0	80.1
rpl32-ndhF_p4	ATTTCACCATGGCGGCTAAT	ACACATTCCGACAAGTTCCC	45.0	50.0	57.9	58.1	2038-2064 bp	1.0	79.9
rpl32-ndhF_p5	ATTTCACCATGGCGGCTAAT	CACATTCCGACAAGTTCCCA	45.0	50.0	57.9	58.1	2037-2063 bp	1.0	79.9