Hotspot

psbZ-trnS-UGA

LSC · 69692-70068 · 377 bp

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Pi 0.0069

MarkerSeek score 56.50

Primer available yes

Label psbZ-trnS-UGA

Feature Metadata

label_name: psbZ-trnS-UGA
feature_type: igs
region: LSC
length_bp: 377
variable_sites: 5
pi: 0.0069
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnS-UGA_p1	CCAACCATCAGGAGAAGCAA	CAACCACTCAGCCATCTCTC	50.0	55.0	57.8	58.0	462-497 bp	1.0	83.0
psbZ-trnS-UGA_p2	CCAACCATCAGGAGAAGCAA	GAGCTATCAACCACTCAGCC	50.0	55.0	57.8	58.1	469-504 bp	1.0	82.7
psbZ-trnS-UGA_p3	CCAACCATCAGGAGAAGCAA	GGAGCTATCAACCACTCAGC	50.0	55.0	57.8	58.1	470-505 bp	1.0	82.7
psbZ-trnS-UGA_p4	CCAACCATCAGGAGAAGCAA	CAAGACCGGAGCTATCAACC	50.0	55.0	57.8	58.1	477-512 bp	1.0	82.7
psbZ-trnS-UGA_p5	GACCAACCATCAGGAGAAGC	CAACCACTCAGCCATCTCTC	55.0	55.0	58.3	58.0	464-499 bp	1.0	82.6