Hotspot

trnD-GUC-trnY-GUA

LSC · 64635-64985 · 351 bp

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Pi 0.0182

MarkerSeek score 59.80

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 351
variable_sites: 13
pi: 0.0182
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	AATTCTTCCTGGGTCGATGC	50.0	50.0	58.5	58.0	456-494 bp	1.0	82.0
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	423-461 bp	1.0	79.7
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	422-460 bp	1.0	79.5
trnD-GUC-trnY-GUA_p4	GGTGCTCTGACCAATTGAAC	AATTCTTCCTGGGTCGATGC	50.0	50.0	57.0	58.0	455-493 bp	1.0	77.9
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	TGGGGACGGACTGTAAATTC	50.0	50.0	58.5	57.2	424-462 bp	1.0	76.3