Hotspot

rpoB-trnC-GCA

LSC · 61096-62264 · 1169 bp

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Pi 0.00513

MarkerSeek score 41.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1169
variable_sites: 11
pi: 0.00513
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTTCTCAAGTTCTTCCGCCA	AAAGGATTTGCAGTCCCCTG	50.0	50.0	57.8	58.1	1323-1339 bp	1.0	78.7
rpoB-trnC-GCA_p2	TCTCAAGTTCTTCCGCCAAG	AAAGGATTTGCAGTCCCCTG	50.0	50.0	57.8	58.1	1321-1337 bp	1.0	78.7
rpoB-trnC-GCA_p3	ACTTCTCAAGTTCTTCCGCC	AAAGGATTTGCAGTCCCCTG	50.0	50.0	57.8	58.1	1324-1340 bp	1.0	78.7
rpoB-trnC-GCA_p4	TTCCGCCAAGCCTTGATTAA	AAAGGATTTGCAGTCCCCTG	45.0	50.0	57.8	58.1	1311-1327 bp	1.0	78.4
rpoB-trnC-GCA_p5	CTTCCGCCAAGCCTTGATTA	AAAGGATTTGCAGTCCCCTG	50.0	50.0	58.2	58.1	1312-1328 bp	1.0	78.1