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rps2-rpoC2

LSC · 50703-51007 · 305 bp

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Pi 0.0193

MarkerSeek score 51.70

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 305
variable_sites: 11
pi: 0.0193
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	ACCAAAATGAACTCCCGCTT	GAGACCCCGTTTACCCCTAT	45.0	55.0	58.0	58.2	396-401 bp	1.0	81.6
rps2-rpoC2_p2	ACCAAAATGAACTCCCGCTT	AGACCCCGTTTACCCCTATT	45.0	50.0	58.0	57.7	395-400 bp	1.0	81.0
rps2-rpoC2_p3	ACCAAAATGAACTCCCGCTT	CATCAGAGACCCCGTTTACC	45.0	55.0	58.0	57.7	401-406 bp	1.0	80.6
rps2-rpoC2_p4	ACCAAAATGAACTCCCGCTT	GAAACATCAGAGACCCCGTT	45.0	50.0	58.0	57.5	405-410 bp	1.0	79.3
rps2-rpoC2_p5	ACCAAAATGAACTCCCGCTT	TGAAACATCAGAGACCCCGT	45.0	50.0	58.0	58.7	406-411 bp	1.0	77.9