Hotspot

ycf3-psaA

LSC · 38329-38932 · 604 bp

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Pi 0.00364

MarkerSeek score 54.10

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 604
variable_sites: 4
pi: 0.00364
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	TGGTTTGATCAAGCTGCTGA	GGGGCCCTTAGCTATTGTTC	45.0	55.0	57.7	58.0	834-838 bp	1.0	78.2
ycf3-psaA_p2	TGGTTTGATCAAGCTGCTGA	GTCTGGCCCATTCCTCAAAA	45.0	50.0	57.7	58.1	799-803 bp	1.0	77.8
ycf3-psaA_p3	GCTATAGCGCTTACTCCAGG	GGGGCCCTTAGCTATTGTTC	55.0	55.0	57.6	58.0	801-805 bp	1.0	77.6
ycf3-psaA_p4	GCTATAGCGCTTACTCCAGG	GTCTGGCCCATTCCTCAAAA	55.0	50.0	57.6	58.1	766-770 bp	1.0	77.2
ycf3-psaA_p5	TGGTTTGATCAAGCTGCTGA	GGTTCCGACGGACGAATAAT	45.0	50.0	57.7	57.8	737-741 bp	1.0	76.7